The degenerate primers CK6-5′ and ARK7-3′ (Palumbi, 1996) were initially used to amplify across an intron in the Ark locus. As these primers were not specific, the different bands obtained were cloned ...

In this paper, we use the mitochondrial region cox1 to assess the usefulness of this marker in addressing evolutionary relationships within the tribe Orchideae. Despite the low overall variation ...

PCR amplification was performed in a total volume of 10 μl, containing 1 μl cDNA, 1 μl 10 × ExTaq buffer (Takara Bio, Inc., Shiga, Japan), 0.25 U ExTaq polymerase (Takara Bio, Inc.), 500 nM each ...

We are far from understanding all the rules that govern the process of RNA splicing and defining the sequence information that governs intron definition. In the September 25 Proceedings of the ...

The sequences of nonsense DNA that interrupt genes could be far more important to the evolution of genomes than previously thought, according to researchers. Their study of the model organism Daphnia ...

BLOOMINGTON, Ind. -- The sequences of nonsense DNA that interrupt genes could be far more important to the evolution of genomes than previously thought, according to a recent Science report by Indiana ...

Plant Systematics and Evolution, Vol. 282, No. 3/4, Special Issue: Molecular evolution and phylogenetic utility of non-coding DNA (October 2009), pp. 201-228 (28 pages) Malpighiales are one of the ...

Within multi-exon genes, after introns are removed, the exons must be spliced back together. Splice-enhancer domains (exon sequences near the intron–exon boundary) help to ensure that this happens ...

A novel type of “jumping gene” may explain why the genomes of complex cells aren’t all equally stuffed with noncoding sequences. All animals, plants, fungi and protists — which collectively make up ...

The human genome is just over 6 feet 8 inches long, which is 2 inches taller than the average NBA basketball player and in total, a lot of nucleotides! But what if the amount of sequence diversity ...